Publications

Failure to reorganize the endoplasmic reticulum (ER) in mitosis results in chromosome missegregation. Here, we show that accurate chromosome segregation in human cells requires cell cycle-regulated ER membrane production. Excess ER membranes increase the viscosity of the mitotic cytoplasm to physically restrict chromosome movements, which impedes the correction of mitotic errors leading to the formation of micronuclei. Mechanistically, we demonstrate that the protein phosphatase CTDNEP1 counteracts mTOR kinase to establish a dephosphorylated pool of the phosphatidic acid phosphatase lipin 1 in interphase. CTDNEP1 control of lipin 1 limits the synthesis of fatty acids for ER membrane biogenesis in interphase that then protects against chromosome missegregation in mitosis. Thus, regulation of ER size can dictate the biophysical properties of mitotic cells, providing an explanation for why ER reorganization is necessary for mitotic fidelity. Our data further suggest that dysregulated lipid metabolism is a potential source of aneuploidy in cancer cells.

Gauge symmetries play a key role in physics appearing in areas such as quantum field theories of the fundamental particles and emergent degrees of freedom in quantum materials. Motivated by the desire to efficiently simulate many-body quantum systems with exact local gauge invariance, gauge equivariant neural-network quantum states are introduced, which exactly satisfy the local Hilbert space constraints necessary for the description of quantum lattice gauge theory with Zd gauge group on different geometries. Focusing on the special case of Z2 gauge group on a periodically identified square lattice, the equivariant architecture is analytically shown to contain the loop-gas solution as a special case. Gauge equivariant neural-network quantum states are used in combination with variational quantum Monte Carlo to obtain compact descriptions of the ground state wavefunction for the Z2 theory away from the exactly solvable limit, and to demonstrate the confining/deconfining phase transition of the Wilson loop order parameter.

We present radio observations (1--40 GHz) for 36 classical novae, representing data from over five decades compiled from the literature, telescope archives, and our own programs. Our targets display a striking diversity in their optical parameters (e.g., spanning optical fading timescales, t_2 = 1--263 days), and we find a similar diversity in the radio light curves. Using a brightness temperature analysis, we find that radio emission from novae is a mixture of thermal and synchrotron emission, with non-thermal emission observed at earlier times. We identify high brightness temperature emission (T_B > 5x10^4 K) as an indication of synchrotron emission in at least 9 (25%) of the novae. We find a class of synchrotron-dominated novae with mildly evolved companions, exemplified by V5589 Sgr and V392 Per, that appear to be a bridge between classical novae with dwarf companions and symbiotic binaries with giant companions. Four of the novae in our sample have two distinct radio maxima (the first dominated by synchrotron and the later by thermal emission), and in four cases the early synchrotron peak is temporally coincident with a dramatic dip in the optical light curve, hinting at a common site for particle acceleration and dust formation. We publish the light curves as tables and encourage use of these data by the broader community in multi-wavelength studies and modeling efforts.

Planetary engulfment events have long been proposed as a lithium (Li) enrichment mechanism contributing to the population of Li-rich giants (A(Li) ≥ 1.5 dex). Using MESA stellar models and A(Li) abundance measurements obtained by the GALAH survey, we calculate the strength and observability of the surface Li enrichment signature produced by the engulfment of a hot Jupiter (HJ). We consider solar-metallicity stars in the mass range of 1–2 M⊙ and the Li supplied by a HJ of 1.0 MJ. We explore engulfment events that occur near the main-sequence turn-off (MSTO) and out to orbital separations of R⋆ ∼ 0.1 au = 22 R⊙. We map our results onto the Hertzsprung–Russell Diagram, revealing the statistical significance and survival time of Li enrichment. We identify the parameter space of masses and evolutionary phases where the engulfment of a HJ can lead to Li enrichment signatures at a 5σ confidence level and with meteoritic abundance strengths. The most compelling strengths and survival times of engulfment-derived Li enrichment are found among host stars of 1.4 M⊙ near the MSTO. Our calculations indicate that planetary engulfment is not a viable enrichment pathway for stars that have evolved beyond the subgiant branch. For these sources, observed Li enhancements are likely to be produced by other mechanisms, such as the Cameron–Fowler process or the accretion of material from an asymptotic giant branch companion. Our results do not account for second-order effects, such as extra mixing processes, which can further dilute Li enrichment signatures.

We introduce the AbacusHOD model and present two applications of AbacusHOD and the AbacusSummit simulations to observations. AbacusHOD is an HOD framework written in Python that is particle-based, multi-tracer, highly generalized, and highly efficient. It is designed specifically with multi-tracer/cosmology analyses for next generation large-scale structure surveys in mind, and takes advantage of the volume and precision offered by the new state-of-the-art AbacusSummit cosmological simulations. The model is also highly customizable and should be broadly applicable to any upcoming surveys and a diverse range of cosmological analyses. In this paper, we demonstrate the capabilities of the AbacusHOD framework through two example applications. The first example demonstrates the high efficiency and the large HOD extension feature set through an analysis full-shape redshift-space clustering of BOSS galaxies at intermediate to small scales (<30Mpc/h), assessing the necessity of introducing secondary galaxy biases (assembly bias). We find strong evidence for using halo environment instead of concentration to trace secondary galaxy bias, a result which also leads to a moderate reduction to the "lensing is low" tension. The second example demonstrates the multi-tracer capabilities of the AbacusHOD package through an analysis of the extended Baryon Oscillation Spectroscopic Survey (eBOSS) cross-correlation measurements between three different galaxy tracers, LRGs, ELGs, and QSOs. We expect the AbacusHOD framework, in combination with the AbacusSummit simulation suite, to play an important role in a simulation-based analysis of the up-coming Dark Energy Spectroscopic Instrument (DESI) datasets.

The periodic pulsations of stars teach us about their underlying physical process. We present a convolutional autoencoder-based pipeline as an automatic approach to search for out-of-distribution anomalous periodic variables within The Zwicky Transient Facility Catalog of Periodic Variable Stars (ZTF CPVS). We use an isolation forest to rank each periodic variable by its anomaly score. Our overall most anomalous events have a unique physical origin: they are mostly highly variable and irregular evolved stars. Multiwavelength data suggest that they are most likely Red Giant or Asymptotic Giant Branch stars concentrated in the Milky Way galactic disk. Furthermore, we show how the learned latent features can be used for the classification of periodic variables through a hierarchical random forest. This novel semi-supervised approach allows astronomers to identify the most anomalous events within a given physical class, significantly increasing the potential for scientific discovery.

We report a significant detection of the hot intergalactic medium in the filamentary bridge connecting the galaxy clusters Abell 399 and Abell 401. This result is enabled by a low-noise, high-resolution map of the thermal Sunyaev-Zeldovich signal from the Atacama Cosmology Telescope (ACT) and Planck satellite. The ACT data provide the 1.65′ resolution that allows us to clearly separate the profiles of the clusters, whose centres are separated by 37′, from the gas associated with the filament. A model that fits for only the two clusters is ruled out compared to one that includes a bridge component at >5σ. Using a gas temperature determined from Suzaku X-ray data, we infer a total mass of (3.3±0.7)×1014M⊙ associated with the filament, comprising about 8% of the entire Abell 399-Abell 401 system. We fit two phenomenological models to the filamentary structure; the favoured model has a width transverse to the axis joining the clusters of ∼1.9Mpc. When combined with the Suzaku data, we find a gas density of (0.88±0.24)×10−4cm−3, considerably lower than previously reported. We show that this can be fully explained by a geometry in which the axis joining Abell 399 and Abell 401 has a large component along the line of sight, such that the distance between the clusters is significantly greater than the 3.2Mpc projected separation on the plane of the sky. Finally, we present initial results from higher resolution (12.7" effective) imaging of the bridge with the MUSTANG-2 receiver on the Green Bank Telescope.

Mitochondrial metabolism is of central importance to diverse aspects of cell and developmental biology. Defects in mitochondria are associated with many diseases, including cancer, neuropathology, and infertility. Our understanding of mitochondrial metabolism in situ and dysfunction in diseases are limited by the lack of techniques to measure mitochondrial metabolic fluxes with sufficient spatiotemporal resolution. Herein, we developed a new method to infer mitochondrial metabolic fluxes in living cells with subcellular resolution from fluorescence lifetime imaging of NADH. This result is based on the use of a generic coarse-grained NADH redox model. We tested the model in mouse oocytes and human tissue culture cells subject to a wide variety of perturbations by comparing predicted fluxes through the electron transport chain (ETC) to direct measurements of oxygen consumption rate. Interpreting the fluorescence lifetime imaging microscopy measurements of NADH using this model, we discovered a homeostasis of ETC flux in mouse oocytes: perturbations of nutrient supply and energy demand of the cell do not change ETC flux despite significantly impacting NADH metabolic state. Furthermore, we observed a subcellular spatial gradient of ETC flux in mouse oocytes and found that this gradient is primarily a result of a spatially heterogeneous mitochondrial proton leak. We concluded from these observations that ETC flux in mouse oocytes is not controlled by energy demand or supply, but by the intrinsic rates of mitochondrial respiration.

The Laplace-Beltrami problem on closed surfaces embedded in three dimensions arises in many areas of physics, including molecular dynamics (surface diffusion), electromagnetics (harmonic vector fields), and fluid dynamics (vesicle deformation). Using classical potential theory,the Laplace-Beltrami operator can be pre-/post-conditioned with integral operators whose kernel is translation invariant, resulting in well-conditioned Fredholm integral equations of the second-kind. These equations have the standard Laplace kernel from potential theory, and therefore the equations can be solved rapidly and accurately using a combination of fast multipole methods (FMMs) and high-order quadrature corrections. In this work we detail such a scheme, presenting two alternative integral formulations of the Laplace-Beltrami problem, each of whose solution can be obtained via FMM acceleration. We then present several applications of the solvers, focusing on the computation of what are known as harmonic vector fields, relevant for many applications in electromagnetics. A battery of numerical results are presented for each application, detailing the performance of the solver in various geometries.

The brain effortlessly solves blind source separation (BSS) problems, but the algorithm it uses remains elusive. In signal processing, linear BSS problems are often solved by Independent Component Analysis (ICA). To serve as a model of a biological circuit, the ICA neural network (NN) must satisfy at least the following requirements: 1. The algorithm must operate in the online setting where data samples are streamed one at a time, and the NN computes the sources on the fly without storing any significant fraction of the data in memory. 2. The synaptic weight update is local, i.e., it depends only on the biophysical variables present in the vicinity of a synapse. Here, we propose a novel objective function for ICA from which we derive a biologically plausible NN, including both the neural architecture and the synaptic learning rules. Interestingly, our algorithm relies on modulating synaptic plasticity by the total activity of the output neurons. In the brain, this could be accomplished by neuromodulators, extracellular calcium, local field potential, or nitric oxide.