Mouse Phenotyping Request for Applications

To maximize the ability to make direct comparisons among mouse models, this request for applications sought applications for systematic, rigorous and scalable analyses of behavioral phenotypes across multiple mouse models. The data produced in this effort was later made available.

SFARI has prioritized a small number of mouse models that have been generated with targeted genetic mutations based on strong human genetic evidence implicating them in autism spectrum disorders. These include mouse models of 16p11.2 chromosomal segment deletion, Cntnap2-/-and Shank3b-/-. Investigators are encouraged to use these models, but SFARI will consider others if compelling reasons based on human genetic data are specified in the application.

In recognition of other ongoing efforts in the research community, SFARI worked to increase availability of select mouse models at Jackson Laboratories. At the time, mouse models of the deletion of the human chromosomal segment 16p11.2 and its reciprocal duplication were available, but SFARI worked to facilitate the availability of additional autism models over time. Investigators were encouraged to use this resource and to contact the foundation for information about availability of additional models.

To achieve the goals of a comprehensive and systematically collected dataset, we expected investigators to test at least three genetic models in the same background strain (C57BL/6). In addition, investigators needed to analyze the behavior of one of the selected autism mouse models in a different genetic background, such as a hybrid background from two inbred strains.