Speaker: Carsen Stringer, Janelia Lab
Topic: Cellpose and Suite2p: anatomical and functional algorithms for cellular segmentation
Abstract: Many biological applications require the segmentation of cell bodies, membranes and nuclei from microscopy images. We therefore developed a generalist, deep learning-based segmentation algorithm called Cellpose, which can very precisely segment a wide range of image types out-of-the-box and does not require model retraining or parameter adjustments. Additionally, many scientists acquire functional imaging data of neural activity. We developed Suite2p, a functional segmentation algorithm, that performs well on a variety of functional imaging data. We will cover how each of these segmentation algorithms work, and the various circumstances where each algorithm performs well.