Invitation Only
Speaker: William Wan, Ph.D., Vanderbilt University
Title: Real Space Approaches to Subtomogram Averaging in STOPGAP
Cryo-electron tomography (cryo-ET) allows for direct 3D reconstruction of specimen areas, allowing for molecular-resolution imaging of complex biological specimens. Subtomogram averaging (STA) is the 3D analog to single particle analysis (SPA), which allows for high-resolution structure determination and characterization of repeating molecular assemblies. My lab develops STOPGAP, an open-source software package to perform STA using real space correlation-based approaches. In this talk, I will go over the fundamental problems in cryo-ET and STA and the approaches we developed in STOPGAP to help overcome them. I will also discuss our recent developments in using our STA algorithms for geometry-based tilt-series refinement to improve the quality of tomograms and subsequent STA.